PMID:17891401
| Citation |
Chung, KJ, Hwang, SK, Hahn, BS, Kim, KH, Kim, JB, Kim, YH, Yang, JS and Ha, SH (2008) Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis. Plant Cell Rep. 27:29-37 |
|---|---|
| Abstract |
The Perilla (Perilla frutescens L. cv. Okdong) oleosin gene, PfOle19, produces a 19-kDa protein that is highly expressed only in seeds. The activity of the -2,015 bp 5'-upstream promoter region of this gene was investigated in transgenic Arabidopsis plants using the fusion reporter constructs of enhanced green fluorescent protein (EGFP) and beta-glucuronidase (GUS). The PfOle19 promoter directs Egfp expression in developing siliques, but not in leaves, stems or roots. In the transgenic Arabidopsis, EGFP fluorescence and histochemical GUS staining were restricted to early seedlings, indehiscent siliques and mature seeds. Progressive 5'-deletions up to the -963 bp position of the PfOle19 promoter increases the spatial control of the gene expression in seeds, but reduces its quantitative levels of expression. Moreover, the activity of the PfOle19 promoter in mature seeds is 4- and 5-fold greater than that of the cauliflower mosaic virus 35S promoter in terms of both EGFP intensity and fluorometric GUS activity, respectively. |
| Links |
PubMed Online version:10.1007/s00299-007-0440-6 |
| Keywords |
Arabidopsis/genetics; Arabidopsis/metabolism; Blotting, Northern; Blotting, Western; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Models, Genetic; Perilla/genetics; Plants, Genetically Modified/genetics; Plants, Genetically Modified/metabolism; Promoter Regions, Genetic/genetics; Reverse Transcriptase Polymerase Chain Reaction; Seeds/genetics; Seeds/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.