PMID:17846588
| Citation |
Kim, I, Kim, J, Min, B, Lee, C and Park, C (2007) Screening of genes related to methylglyoxal susceptibility. J. Microbiol. 45:339-43 |
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| Abstract |
Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient strain. In addition to its MG sensitivity, the insertion in yqiI exhibited an impaired motility resulting from a reduced flagellar expression. |
| Links | |
| Keywords |
Acetone/analogs & derivatives; Acetone/chemistry; Acetone/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Flagella/genetics; Flagella/physiology; Genes, Bacterial/genetics; Genome, Bacterial; Lactic Acid/chemistry; Lactic Acid/metabolism; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Models, Genetic; Mutagenesis, Insertional/drug effects; Mutation/drug effects; Pyruvaldehyde/pharmacology |
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