PMID:1783373
| Citation |
Griffin, LD, Gelb, BD, Wheeler, DA, Davison, D, Adams, V and McCabe, ER (1991) Mammalian hexokinase 1: evolutionary conservation and structure to function analysis. Genomics 11:1014-24 |
|---|---|
| Abstract |
We have amplified and sequenced the complete coding region of bovine hexokinase isoenzyme 1 (HK1) from brain RNA with PCR primers selected for sequence conservation. The sequence information was analyzed to evaluate the evolutionary and structure-function relationships among the mammalian and yeast HK isoenzymes. Structure to function analysis identified an unduplicated, invariant N-terminal domain involved in HK1 outer mitochondrial membrane targeting, as well as putative carbohydrate and nucleotide-binding sites in the regulatory and catalytic halves of HK1 essential to enzyme function. The ATP-binding site in the catalytic half of the HK1 protein resembles nucleotide-binding regions from protein kinases, with the single amino acid replacement (lysine to glutamate) in the ATP-binding site of the amino half explaining the loss of HK1 catalytic function in the regulatory domain. Sequence comparisons suggest that the 50-kDa mammalian and yeast glucokinases arose separately in evolution. In addition to providing valuable phylogenetic and structure-function insights, this work provides an efficient strategy for rapid cloning and sequencing of the coding regions for other HKs and related proteins. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Binding Sites; Biological Evolution; Cattle; Cloning, Molecular; Hexokinase/chemistry; Hexokinase/genetics; Hexokinase/metabolism; Humans; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Molecular Sequence Data; Phylogeny; Rats; Sequence Homology, Nucleic Acid; Structure-Activity Relationship |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.