PMID:17768349

Citation	Arai, T, Watanabe, S, Matsumi, R, Atomi, H, Imanaka, T and Miki, K (2007) Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63:765-7
Abstract	The hydrogenase maturation protein HypE is involved in the biosynthesis of the CN ligands of the active-site iron of [NiFe] hydrogenases using carbamoylphosphate as a substrate. Here, the crystallization and preliminary crystallographic analysis of HypE from Thermococcus kodakaraensis KOD1 are reported. Crystals of HypE (338 amino acids, 35.9 kDa) have been obtained by the sitting-drop vapour-diffusion method using 2-methyl-2,4-pentanediol (MPD) as a precipitant. The crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 88.3, b = 45.8, c = 75.1 A. There is one HypE molecule in the asymmetric unit. A complete native X-ray diffraction data set was collected to a maximum resolution of 1.55 A at 100 K.
Links	PubMed PMC2376326 Online version:10.1107/S1744309107038833
Keywords	Bacterial Proteins/chemistry; Bacterial Proteins/isolation & purification; Cloning, Molecular; Crystallization; Crystallography, X-Ray; Hydrogenase/chemistry; Hydrogenase/isolation & purification; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Restriction Mapping; Thermococcus/enzymology
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