PMID:17698959
| Citation |
Loiseau, L, Gerez, C, Bekker, M, Ollagnier-de Choudens, S, Py, B, Sanakis, Y, Teixeira de Mattos, J, Fontecave, M and Barras, F (2007) ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 104:13626-31 |
|---|---|
| Abstract |
Understanding the biogenesis of iron-sulfur (Fe-S) proteins is relevant to many fields, including bioenergetics, gene regulation, and cancer research. Several multiprotein complexes assisting Fe-S assembly have been identified in both prokaryotes and eukaryotes. Here, we identify in Escherichia coli an A-type Fe-S protein that we named ErpA. Remarkably, erpA was found essential for growth of E. coli in the presence of oxygen or alternative electron acceptors. It was concluded that isoprenoid biosynthesis was impaired by the erpA mutation. First, the eukaryotic mevalonate-dependent pathway for biosynthesis of isopentenyl diphosphate restored the respiratory defects of an erpA mutant. Second, the erpA mutant contained a greatly reduced amount of ubiquinone and menaquinone. Third, ErpA bound Fe-S clusters and transferred them to apo-IspG, a protein catalyzing isopentenyl diphosphate biosynthesis in E. coli. Surprisingly, the erpA gene maps at a distance from any other Fe-S biogenesis-related gene. ErpA is an A-type Fe-S protein that is characterized by an essential role in cellular metabolism. |
| Links |
PubMed PMC1959432 Online version:10.1073/pnas.0705829104 |
| Keywords |
Aerobiosis; Anaerobiosis; Benzoquinones/metabolism; Cell Respiration; Escherichia coli/cytology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/classification; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Iron-Sulfur Proteins/classification; Iron-Sulfur Proteins/genetics; Iron-Sulfur Proteins/metabolism; Mevalonic Acid/pharmacology; Microbial Viability; Multigene Family; Mutation/genetics; Phenotype; Protein Binding |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.