PMID:17693520
| Citation |
Scheffers, DJ, Robichon, C, Haan, GJ, den Blaauwen, T, Koningstein, G, van Bloois, E, Beckwith, J and Luirink, J (2007) Contribution of the FtsQ transmembrane segment to localization to the cell division site. J. Bacteriol. 189:7273-80 |
|---|---|
| Abstract |
The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement an ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. |
| Links |
PubMed PMC2168451 Online version:10.1128/JB.00723-07 |
| Keywords |
Amino Acid Substitution/genetics; Artificial Gene Fusion; Cell Cycle Proteins/metabolism; Cell Division/genetics; Cell Division/physiology; Cell Membrane/chemistry; DNA Mutational Analysis; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Genes, Reporter; Genetic Complementation Test; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Microscopy, Fluorescence; Mutation; Protein Binding; Protein Structure, Tertiary/genetics; Protein Structure, Tertiary/physiology; Sequence Deletion/genetics |
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