PMID:17686457
| Citation |
Scortecci, KC, Lima, AF, Carvalho, FM, Silva, UB, Agnez-Lima, LF and Batistuzzo de Medeiros, SR (2007) A characterization of a MutM/Fpg ortholog in sugarcane--A monocot plant. Biochem. Biophys. Res. Commun. 361:1054-60 |
|---|---|
| Abstract |
Plant genomic projects, such as Arabidopsis thaliana, rice, and maize, have provided excellent tools for comparative genome analysis on Base Excision DNA Repair (BER). A data-mining study associated with the SUCEST Genome project identified two EST clusters that shared homology to the bacteria MutM/Fpg protein. Comparative analyses presented here show a duplication of the MutM/Fpg gene in sugarcane, wheat and rice. The complementation assays show that both cDNAs from sugarcane are able to complement the Fpg and MutY-glycosylase deficiency in a double mutant Escherichia coli strain (CC104mutMmutY), reducing the spontaneous mutation frequency by 10-fold. The expression analyses by semi-quantitative RT-PCR show that these two mRNAs have different expression levels. |
| Links |
PubMed Online version:10.1016/j.bbrc.2007.07.134 |
| Keywords |
Amino Acid Sequence; Computational Biology; DNA-Formamidopyrimidine Glycosylase/chemistry; DNA-Formamidopyrimidine Glycosylase/classification; DNA-Formamidopyrimidine Glycosylase/genetics; Escherichia coli/genetics; Gene Expression; Genetic Complementation Test; Molecular Sequence Data; Phylogeny; Plant Proteins/chemistry; Plant Proteins/classification; Plant Proteins/genetics; Saccharum/enzymology; Saccharum/genetics; Sequence Alignment |
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