PMID:17686166
| Citation |
Tannu, NS and Hemby, SE (2007) De novo protein sequence analysis of Macaca mulatta. BMC Genomics 8:270 |
|---|---|
| Abstract |
Macaca mulatta is one of the most utilized non-human primate species in biomedical research offering unique behavioral, neuroanatomical, and neurobiochemcial similarities to humans. This makes it a unique organism to model various diseases such as psychiatric and neurodegenerative illnesses while also providing insight into the complexities of the primate brain. A major obstacle in utilizing rhesus monkey models for human disease is the paucity of protein annotations for this species (~42,000 protein annotations) compared to 330,210 protein annotations for humans. The lack of available information limits the use of rhesus monkey for proteomic scale studies which rely heavily on database searches for protein identification. While characterization of proteins of interest from Macaca mulatta using the standard database search engines (e.g., MASCOT) can be accomplished, searches must be performed using a 'broad species database' which does not provide optimal confidence in protein annotation. Therefore, it becomes necessary to determine partial or complete amino acid sequences using either manual or automated de novo peptide sequence analysis methods. |
| Links |
PubMed PMC1965481 Online version:10.1186/1471-2164-8-270 |
| Keywords |
Amino Acid Sequence; Animals; Electrophoresis, Gel, Two-Dimensional; Macaca mulatta; Male; Models, Biological; Molecular Sequence Data; Organometallic Compounds/pharmacology; Proteomics/methods; Sequence Analysis, Protein/methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling/methods |
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