PMID:17673179
| Citation |
Jiang, PX, Wang, J, Feng, Y and He, ZG (2007) Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2. Biochem. Biophys. Res. Commun. 361:651-8 |
|---|---|
| Abstract |
The Cdc6 protein has been suggested as a loader for the eukaryotic MCM helicase. Archaeal replication machinery represents a core version of that in eukaryotes. In the current work, three eukaryotic Orc1/Cdc6 homologs (SsoCdc6-1, -2, and -3) from crenarchaeon Sulfolobus solfataricus were shown to have totally different effects on the interactions with SsoMCM helicase. SsoCdc6-2 stimulates the binding of the SsoMCM onto the origin DNA, but SsoCdc6-1 and SsoCdc6-3 significantly inhibit the loading activities, and these inhibitive effects can not be reversed by the stimulation of SsoCdc6-2. Using pull-down assays, we showed that three SsoCdc6 proteins interacted physically with the SsoMCM. Furthermore, the C-terminal domains of SsoCdc6 proteins were shown to physically and functionally affect the interactions with SsoMCM. This is the first report on the divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on regulating the loading of the MCM helicase onto the origins in the archaeon. |
| Links |
PubMed Online version:10.1016/j.bbrc.2007.07.073 |
| Keywords |
Archaeal Proteins/metabolism; Base Sequence; Binding Sites; Cell Cycle Proteins/metabolism; DNA/metabolism; DNA Helicases/metabolism; DNA Replication; Models, Genetic; Molecular Sequence Data; Origin Recognition Complex/metabolism; Replication Origin; Sulfolobus solfataricus/enzymology; Sulfolobus solfataricus/genetics; Sulfolobus solfataricus/metabolism |
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