PMID:1764518

Citation	Zengel, JM and Lindahl, L (1991) Ribosomal protein L4 of Escherichia coli: in vitro analysis of L4-mediated attenuation control. Biochimie 73:719-27
Abstract	Ribosomal protein L4 of Escherichia coli functions not only as a component of the ribosome but also as a regulatory factor inhibiting both transcription and translation of its own operon, the 11 gene S10 operon. L4-mediated transcription control results in premature termination of transcription within the 172 base S10 operon leader. This attenuation control can be reproduced in a purified transcription system containing RNA polymerase, but depends on the addition of transcription factor NusA. The NusA stimulation saturates at about 2-4 copies per RNA polymerase. The L4 effect plateaus at about 4 copies per RNA polymerase. The specific recognition sites on 23S rRNA and in the S10 leader for L4 binding are not yet known. However, we can demonstrate that a fragment of 23S rRNA containing the proximal 840 bases can eliminate in vitro L4-stimulated attenuation, and hence, contains the information sufficient for L4 binding to 23S rRNA.
Links	PubMed
Keywords	Base Sequence; Binding Sites; DNA Mutational Analysis; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Nucleic Acid Conformation; Operon/genetics; RNA, Ribosomal, 16S/metabolism; RNA, Ribosomal, 23S/chemistry; Ribosomal Proteins/metabolism; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.