PMID:17640895

Citation	Bougdour, A and Gottesman, S (2007) ppGpp regulation of RpoS degradation via anti-adaptor protein IraP. Proc. Natl. Acad. Sci. U.S.A. 104:12896-901
Abstract	IraP is a small protein that interferes with the delivery of sigma(S) (RpoS) to the ClpXP protease by blocking the action of RssB, an adaptor protein for sigma(S) degradation. IraP was previously shown to mediate stabilization of sigma(S) during phosphate starvation. Here, we show that iraP is transcribed in response to phosphate starvation; this response is mediated by ppGpp. The iraP promoter is positively regulated by ppGpp, dependent on the discriminator region of the iraP promoter. Sensing of phosphate starvation requires SpoT but not RelA. The results demonstrate a target for positive regulation by ppGpp and suggest that the cell use of ppGpp to mediate a variety of starvation responses operates in part by modulating sigma(S) levels.
Links	PubMed PMC1937563 Online version:10.1073/pnas.0705561104
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation; Molecular Sequence Data; Mutation/genetics; Phosphates/pharmacology; Pyrophosphatases/metabolism; RNA, Messenger/genetics; Sigma Factor/genetics; Sigma Factor/metabolism
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