PMID:17636125
| Citation |
Tocchini-Valentini, GD, Fruscoloni, P and Tocchini-Valentini, GP (2007) The dawn of dominance by the mature domain in tRNA splicing. Proc. Natl. Acad. Sci. U.S.A. 104:12300-5 |
|---|---|
| Abstract |
The relationship between enzyme architecture and substrate specificity among archaeal pre-tRNA splicing endonucleases has been investigated more deeply, by using biochemical assays and model building. The enzyme from Archeoglobus fulgidus (AF) is particularly interesting: it cleaves the bulge-helix-bulge target without requiring the mature tRNA domain, but, when the target is a bulge-helix-loop, the mature domain is required. A model of AF based on its electrostatic potential shows three polar patches interacting with the pre-tRNA substrate. A simple deletion mutant of the AF endonuclease lacking two of the three polar patches no longer cleaves the bulge-helix-loop substrate with or without the mature domain. This single deletion shows a possible path for the evolution of eukaryal splicing endonucleases from the archaeal enzyme. |
| Links |
PubMed PMC1941465 Online version:10.1073/pnas.0705537104 |
| Keywords |
Amino Acid Sequence; Archaeal Proteins/chemistry; Archaeal Proteins/genetics; Archaeal Proteins/metabolism; Archaeoglobus fulgidus/enzymology; Base Sequence; Binding Sites; Humans; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Protein Binding; Protein Structure, Tertiary; RNA Splicing/genetics; RNA, Transfer/chemistry; RNA, Transfer/genetics; Static Electricity; Structural Homology, Protein; Substrate Specificity |
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