PMID:17612492
| Citation |
Celesnik, H, Deana, A and Belasco, JG (2007) Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal. Mol. Cell 27:79-90 |
|---|---|
| Abstract |
The common belief that endonucleolytic cleavage is the initial, rate-determining step of mRNA decay in Escherichia coli fails to explain the influence of 5' termini on the half-lives of primary transcripts. We have re-examined the initial events of RNA degradation in that organism by devising an assay to probe the 5' phosphorylation state of RNA and by employing a self-cleaving hammerhead ribozyme to investigate the degradative consequences of an unphosphorylated 5' end. These studies have identified a previously unrecognized prior step in decay that triggers subsequent internal cleavage by the endonuclease RNase E and thereby governs RNA longevity: the rate-determining conversion of a triphosphorylated to a monophosphorylated 5' terminus. Our findings redefine the role of RNase E in RNA degradation and explain how unpaired 5'-terminal nucleotides can facilitate access to internal cleavage sites within primary transcripts. Moreover, these results reveal a striking parallel between the mechanisms of mRNA decay in prokaryotic and eukaryotic organisms. |
| Links |
PubMed PMC2196405 Online version:10.1016/j.molcel.2007.05.038 |
| Keywords |
Base Sequence; Diphosphates/isolation & purification; Diphosphates/metabolism; Escherichia coli/genetics; Hydroxyl Radical; Models, Biological; Molecular Sequence Data; Nucleic Acid Conformation; Phosphorylation; RNA Stability; RNA, Bacterial/biosynthesis; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Messenger/biosynthesis; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism |
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