PMID:17578452
| Citation |
Foti, JJ, Persky, NS, Ferullo, DJ and Lovett, ST (2007) Chromosome segregation control by Escherichia coli ObgE GTPase. Mol. Microbiol. 65:569-81 |
|---|---|
| Abstract |
Escherichia coli cells depleted of the conserved GTPase, ObgE, show early chromosome-partitioning defects and accumulate replicated chromosomes in which the terminus regions are colocalized. Cells lacking ObgE continue to initiate replication, with a normal ratio of the origin to terminus. Localization of the SeqA DNA binding protein, normally seen as punctate foci, however, was disturbed. Depletion of ObgE also results in cell filamentation, with polyploid DNA content. Depletion of ObgE did not cause lethality, and cells recovered fully after expression of ObgE was restored. We propose a model in which ObgE is required to license chromosome segregation and subsequent cell cycle events. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2007.05811.x |
| Keywords |
Bacterial Outer Membrane Proteins/analysis; Bacterial Outer Membrane Proteins/metabolism; Chromosome Segregation/genetics; Chromosomes, Bacterial/genetics; DNA-Binding Proteins/analysis; DNA-Binding Proteins/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/analysis; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Gene Deletion; Monomeric GTP-Binding Proteins/genetics; Monomeric GTP-Binding Proteins/physiology; Protein Biosynthesis/genetics; Replication Origin |
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