PMID:17570970
| Citation |
Kim, JW, Chung, PR, Hwang, MK and Choi, EY (2007) Double-stranded RNA virus in Korean isolate IH-2 of Trichomonas vaginalis. Korean J. Parasitol. 45:87-94 |
|---|---|
| Abstract |
In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus. |
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| Keywords |
Abscess/parasitology; Abscess/pathology; Animals; Capsid Proteins/genetics; Cloning, Molecular; Disease Models, Animal; Female; Frameshifting, Ribosomal; Giardiavirus/classification; Giardiavirus/genetics; Giardiavirus/isolation & purification; Humans; Korea; Mice; Molecular Sequence Data; Open Reading Frames; Phylogeny; RNA Replicase/genetics; RNA, Double-Stranded/genetics; RNA, Viral/genetics; Sequence Analysis, DNA; Sequence Homology; Trichomonas Infections/virology; Trichomonas vaginalis/genetics; Trichomonas vaginalis/isolation & purification; Trichomonas vaginalis/pathogenicity; Trichomonas vaginalis/virology; Virulence |
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