PMID:17421044

Citation	Villadsen, J and Patil, KR (2007) Optimal fed-batch cultivation when mass transfer becomes limiting. Biotechnol. Bioeng. 98:706-10
Abstract	In the design of an aerobic fed-batch process to produce, for example, a pharmaceutical protein, the volumetric production rate will eventually become limited by mass transfer when the biomass concentration exceeds a certain upper limit x. It appears to be common practice to switch from exponential feed of substrate to a constant feed rate when x is reached. This is done to avoid oxygen starvation with a potential risk of undesired stress responses. But with a constant feed rate the carbon source (glucose) concentration may decrease to a low level with a resulting loss of viability and an undesired production of endotoxins. It is shown that an exponential feeding strategy may be continued, but with a smaller exponent than the one used before oxygen limitation occurs. This will diminish the potential detrimental effects on the culture due to low glucose concentration, and the total time to reach a given final biomass concentration will be reduced.
Links	PubMed Online version:10.1002/bit.21451
Keywords	Bacteria, Aerobic/physiology; Bioreactors/microbiology; Cell Culture Techniques/methods; Cell Survival; Computer Simulation; Glucose/metabolism; Models, Biological; Oxygen/metabolism
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.