PMID:17404237
| Citation |
Sklar, JG, Wu, T, Gronenberg, LS, Malinverni, JC, Kahne, D and Silhavy, TJ (2007) Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 104:6400-5 |
|---|---|
| Abstract |
A major role of the outer membrane (OM) of Gram-negative bacteria is to provide a protective permeability barrier for the cell, and proper maintenance of the OM is required for cellular viability. OM biogenesis requires the coordinated assembly of constituent lipids and proteins via dedicated OM assembly machineries. We have previously shown that, in Escherichia coli, the multicomponent YaeT complex is responsible for the assembly of OM beta-barrel proteins (OMPs). This complex contains the OMP YaeT and three OM lipoproteins. Here, we report another component of the YaeT complex, the OM lipoprotein small protein A (SmpA). Strains carrying loss-of-function mutations in smpA are viable but exhibit defects in OMP assembly. Biochemical experiments show that SmpA is involved in maintaining complex stability. Taken together, these experiments establish an important role for SmpA in both the structure and function of the YaeT complex. |
| Links |
PubMed PMC1851043 Online version:10.1073/pnas.0701579104 |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Blotting, Western; Chromatography, Affinity; DNA Primers; Escherichia coli/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Immunoprecipitation; Multiprotein Complexes/genetics; Polymerase Chain Reaction; Species Specificity |
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