PMID:1740158
| Citation |
Maras, B, Sweeney, G, Barra, D, Bossa, F and John, RA (1992) The amino acid sequence of glutamate decarboxylase from Escherichia coli. Evolutionary relationship between mammalian and bacterial enzymes. Eur. J. Biochem. 204:93-8 |
|---|---|
| Abstract |
The amino acid sequence of glutamate decarboxylase from Escherichia coli was solved by a combination of automated Edman degradation of peptide fragments derived by proteolytic and chemical cleavage and sequencing of DNA. Correct alignment of three peptides, for which no peptide overlaps were available, was achieved by sequencing a 1.1-kbp fragment of DNA produced by a polymerase-chain reaction using primers corresponding to sequences known to be in amino-terminal and carboxy-terminal regions of the protein. Sequence similarity (24% identity) with mammalian glutamate decarboxylase was found to be limited to a 55-residue sequence around the lysine residue that binds the coenzyme. Stronger similarity (38% identity), again confined to the same region, is seen with bacterial pyridoxal-phosphate-dependent histidine decarboxylase. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Biological Evolution; Cats; DNA, Bacterial/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Glutamate Decarboxylase/chemistry; Glutamate Decarboxylase/genetics; Molecular Sequence Data; Polymerase Chain Reaction; Rats; Restriction Mapping; Sequence Homology, Nucleic Acid |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
