PMID:17384964
| Citation |
Marchi, P, Longhi, V, Zangrossi, S, Gaetani, E, Briani, F and Dehò, G (2007) Autogenous regulation of Escherichia coli polynucleotide phosphorylase during cold acclimation by transcription termination and antitermination. Mol. Genet. Genomics 278:75-84 |
|---|---|
| Abstract |
Adaptation of Escherichia coli at low temperature implicates a drastic reprogramming of gene expression patterns. Mechanisms operating downstream of transcription initiation, such as control of transcription termination, mRNA stability and translatability, play a major role in controlling gene expression in the cold acclimation phase. It was previously shown that Rho-dependent transcription termination within pnp, the gene encoding polynucleotide phosphorylase (PNPase), was suppressed in pnp nonsense mutants, whereas it was restored by complementation with wild type allele. Using a tRNA gene as a reporter and the strong Rho-dependent transcription terminator t ( imm ) of bacteriophage P4 as a tester, here we show that specific sites in the 5'-untranslated region of pnp mRNA are required for PNPase-sensitive cold-induced suppression of Rho-dependent transcription termination. We suggest that suppression of Rho-dependent transcription termination within pnp and its restoration by PNPase is an autogenous regulatory circuit that modulates pnp expression during cold acclimation. |
| Links |
PubMed Online version:10.1007/s00438-007-0231-3 |
| Keywords |
5' Untranslated Regions; Alleles; Base Sequence; Cold Temperature; Escherichia coli/enzymology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Reporter; Molecular Sequence Data; Nucleic Acid Conformation; Plasmids/metabolism; Polyribonucleotide Nucleotidyltransferase/biosynthesis; Polyribonucleotide Nucleotidyltransferase/genetics; RNA, Transfer/chemistry; RNA, Transfer/metabolism; Transcription, Genetic; rho GTP-Binding Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.