PMID:17322400

Citation	Wang, KH, Sauer, RT and Baker, TA (2007) ClpS modulates but is not essential for bacterial N-end rule degradation. Genes Dev. 21:403-8
Abstract	In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation in vivo but rather stimulated turnover in a sequence-specific manner. Although ClpS substantially enhanced degradation of N-end substrates at low substrate concentrations in vitro, it suppressed the degradation rate when substrate was saturating. Thus, we conclude that ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway.
Links	PubMed PMC1804329 Online version:10.1101/gad.1511907
Keywords	Amino Acid Sequence; Bacterial Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Endopeptidase Clp/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Molecular Sequence Data; Substrate Specificity
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