PMID:1731086
| Citation |
Mason, SW, Li, J and Greenblatt, J (1992) Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10. J. Mol. Biol. 223:55-66 |
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| Abstract |
The Escherichia coli proteins NusB and ribosomal protein S10 are important for transcription antitermination by the bacteriophage lambda N protein. We have used sucrose gradient co-sedimentation and affinity chromatography with immobilized ribosomal protein S10, a glutathione S-transferase-S10 fusion protein, and NusB to show that NusB binds directly and very selectively to S10. The interaction is non-ionic and has an estimated Kd value of 10(-7) M. We hypothesize that NusB binds to N-modified transcription complexes primarily by interacting with S10. |
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| Keywords |
Bacterial Proteins/genetics; Chromatography, Affinity; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Mutation; Protein Binding; Recombinant Fusion Proteins/metabolism; Ribosomal Proteins/genetics; Terminator Regions, Genetic; Transcription Factors; Transcription, Genetic |
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