PMID:17292651

Citation	Alper, H and Stephanopoulos, G (2007) Global transcription machinery engineering: a new approach for improving cellular phenotype. Metab. Eng. 9:258-67
Abstract	It is now generally accepted that most cellular phenotypes are affected by many genes. As a result, engineering a desired phenotype would be facilitated enormously by simultaneous multiple gene modification, yet the capacity to introduce such modifications is very limited. Here, we demonstrate that the components of global cellular transcription machinery (specifically, sigma(70)) can be engineered to allow for global perturbations of the transcriptome, which can help unlock complex phenotypes. Results from three distinct phenotypes (ethanol tolerance, metabolite overproduction, and multiple phenotypes) are provided as proof-of-concept. In each case, the tool of global transcription machinery engineering (gTME) outperformed traditional approaches by quickly and more effectively optimizing phenotypes.
Links	PubMed Online version:10.1016/j.ymben.2006.12.002
Keywords	DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial/genetics; Genetic Engineering/methods; Models, Biological; Phenotype; Sigma Factor/genetics; Sigma Factor/metabolism; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Ethanol and SDS tolerance experiments were done in DH5alpha

Accessions	SMD Category	SMD Subcategory	SMD status
edit table

</protect>

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.