PMID:17289755
| Citation |
Choonee, N, Even, S, Zig, L and Putzer, H (2007) Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism. Nucleic Acids Res. 35:1578-88 |
|---|---|
| Abstract |
In contrast to Escherichia coli no molecular mechanism controlling the biosynthesis of ribosomal proteins has been elucidated in Gram-positive organisms. Here we show that the expression of the Bacillus subtilis infC-rpmI-rplT operon encoding translation factor IF3 and the ribosomal proteins L35 and L20 is autoregulated by a complex transcription attenuation mechanism. It implicates a 200-bp leader region upstream of infC which contains two conserved regulatory elements, one of which can act as a transcription terminator. Using in vitro and in vivo approaches we show that expression of the operon is regulated at the level of transcription elongation by a change in the structure of the leader mRNA which depends upon the presence of ribosomal protein L20. L20 binds to a phylogenetically conserved domain and provokes premature transcription termination at the leader terminator. Footprint and toeprint experiments support a regulatory model involving molecular mimicry between the L20-binding sites on 23S rRNA and the mRNA. Our data suggest that Nomura's model of ribosomal protein biosynthesis based on autogenous control and molecular mimicry is also valid in Gram-positive organisms. |
| Links |
PubMed PMC1865079 Online version:10.1093/nar/gkm011 |
| Keywords |
5' Untranslated Regions/chemistry; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Conserved Sequence; Gene Expression Regulation, Bacterial; Models, Genetic; Molecular Mimicry; Molecular Sequence Data; Nucleic Acid Conformation; Operon; Promoter Regions, Genetic; RNA, Messenger/chemistry; RNA, Ribosomal/chemistry; Regulatory Elements, Transcriptional; Ribosomal Proteins/genetics; Ribosomal Proteins/metabolism; Terminator Regions, Genetic; Transcription, Genetic |
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