PMID:17285480
| Citation |
Souza, TM, Abrantes, JL, de A Epifanio, R, Leite Fontes, CF and Frugulhetti, IC (2007) The alkaloid 4-methylaaptamine isolated from the sponge Aaptos aaptos impairs Herpes simplex virus type 1 penetration and immediate-early protein synthesis. Planta Med. 73:200-5 |
|---|---|
| Abstract |
We describe in this paper that the alkaloid 4-methylaaptamine, isolated from the marine sponge Aaptos aaptos, inhibited HSV-1 infection. We initially observed that 4-methylaaptamine inhibited HSV-1 replication in Vero cells in a dose-dependent manner with an EC50 value of 2.4 microM. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, since the CC50 value of 4-methylaaptamine was equal to 72 microM. Next, we found that 4-methylaaptamine sustained antiherpetic activity even when added to HSV-1-infected Vero cells at 4 h after infection, suggesting that this compound inhibits initial events during HSV-1 replication. We observed that 4-methylaaptamine impaired HSV-1 penetration without affecting viral adsorption. In addition, the tested compound could inhibit, in an MOI-dependent manner, the expression of an HSV-1 immediate-early protein, ICP27, thus preventing the inhibition of macromolecular synthesis induced by this virus. Our results warrant further investigation on the pharmacokinetics of 4-methylaaptamine and propose that this alkaloid could be considered as a potential compound for HSV-1 therapy. |
| Links |
PubMed Online version:10.1055/s-2007-967109 |
| Keywords |
Alkaloids/administration & dosage; Alkaloids/pharmacology; Alkaloids/therapeutic use; Animals; Antiviral Agents/administration & dosage; Antiviral Agents/pharmacology; Antiviral Agents/therapeutic use; Cercopithecus aethiops; Dose-Response Relationship, Drug; Herpes Simplex/drug therapy; Herpesvirus 1, Human/drug effects; Herpesvirus 1, Human/metabolism; Microbial Sensitivity Tests; Naphthyridines/administration & dosage; Naphthyridines/pharmacology; Naphthyridines/therapeutic use; Phytotherapy; Plant Extracts/administration & dosage; Plant Extracts/pharmacology; Plant Extracts/therapeutic use; Porifera; Vero Cells; Virus Replication/drug effects |
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