PMID:17272300
| Citation |
Watt, RM, Wang, J, Leong, M, Kung, HF, Cheah, KS, Liu, D, Danchin, A and Huang, JD (2007) Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. Nucleic Acids Res. 35:e37 |
|---|---|
| Abstract |
To investigate the feasibility of conducting a genomic-scale protein labeling and localization study in Escherichia coli, a representative subset of 23 coding DNA sequences (CDSs) was selected for chromosomal tagging with one or more fluorescent protein genes (EGFP, EYFP, mRFP1, DsRed2). We used lambda-Red recombination to precisely and efficiently position PCR-generated DNA targeting cassettes containing a fluorescent protein gene and an antibiotic resistance marker, at the C-termini of the CDSs of interest, creating in-frame fusions under the control of their native promoters. We incorporated cre/loxP and flpe/frt technology to enable multiple rounds of chromosomal tagging events to be performed sequentially with minimal disruption to the target locus, thus allowing sets of proteins to be co-localized within the cell. The visualization of labeled proteins in live E. coli cells using fluorescence microscopy revealed a striking variety of distributions including: membrane and nucleoid association, polar foci and diffuse cytoplasmic localization. Fifty of the fifty-two independent targeting experiments performed were successful, and 21 of the 23 selected CDSs could be fluorescently visualized. Our results show that E. coli has an organized and dynamic proteome, and demonstrate that this approach is applicable for tagging and (co-) localizing CDSs on a genome-wide scale. |
| Links |
PubMed PMC1874593 Online version:10.1093/nar/gkl1158 |
| Keywords |
Chromosomes, Bacterial/chemistry; DNA, Bacterial/chemistry; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/analysis; Escherichia coli Proteins/genetics; Fluorescent Dyes/analysis; Genes, Suppressor; Luminescent Proteins/analysis; Luminescent Proteins/genetics; Methionine Adenosyltransferase/analysis; Methionine Adenosyltransferase/genetics; Microscopy, Fluorescence; Proteome/analysis; Proteome/genetics; Proteomics/methods; Recombinant Fusion Proteins/analysis; Recombination, Genetic; Transferases/analysis; Transferases/genetics |
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