PMID:1722767
| Citation |
Neal, BL, Tsiolis, GC, Heuzenroeder, MW, Manning, PA and Reeves, PR (1991) Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain. FEMS Microbiol. Lett. 66:345-51 |
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| Abstract |
The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K-12 was localised to a 10.7 to 14.15-kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci. |
| Links | |
| Keywords |
Animals; Antigens, Bacterial/genetics; Chickens/microbiology; Chromosome Mapping; Escherichia coli/genetics; Escherichia coli/pathogenicity; Gene Expression Regulation, Bacterial; Genes, Bacterial/genetics; Lipopolysaccharides/analysis; Lipopolysaccharides/genetics; O Antigens; Restriction Mapping; Sequence Homology, Nucleic Acid |
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