PMID:17187078

Citation	Woodward, JJ, Martin, NI and Marletta, MA (2007) An Escherichia coli expression-based method for heme substitution. Nat. Methods 4:43-5
Abstract	Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions.
Links	PubMed Online version:10.1038/nmeth984
Keywords	Chromatography, High Pressure Liquid/methods; Escherichia coli/metabolism; Hemeproteins/chemistry; Hemeproteins/metabolism; Porphyrins/chemistry; Porphyrins/metabolism; Sensitivity and Specificity; Spectrophotometry, Ultraviolet/methods; Time Factors
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