PMID:17183171

Citation	Cartron, ML, Mitchell, SA, Woodhall, MR, Andrews, SC and Watson, KA (2007) Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-secreted periplasmic protein with a potential role in iron transport. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63:37-41
Abstract	YcdB is a periplasmic haem-containing protein from Escherichia coli that has a potential role in iron transport. It is currently the only reported haem-containing Tat-secreted substrate. Here, the overexpression, purification, crystallization and structure determination at 2.0 A resolution are reported for the apo form of the protein. The apo-YcdB structure resembles those of members of the haem-dependent peroxidase family and thus confirms that YcdB is also a member of this family. Haem-soaking experiments with preformed apo-YcdB crystals have been optimized to successfully generate haem-containing YcdB crystals that diffract to 2.9 A. Completion of model building and structure refinement are under way.
Links	PubMed PMC2330106 Online version:10.1107/S174430910604509X
Keywords	Biological Transport/physiology; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Hemeproteins/biosynthesis; Hemeproteins/chemistry; Hemeproteins/genetics; Hemeproteins/metabolism; Iron/metabolism; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/metabolism; Periplasmic Proteins/chemistry; Periplasmic Proteins/metabolism; X-Ray Diffraction
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