PMID:17163457
| Citation |
Mitra, P, Xie, R, Harper, JW, Stein, JL, Stein, GS and van Wijnen, AJ (2007) HiNF-P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription. J. Cell. Biochem. 101:181-91 |
|---|---|
| Abstract |
Cell cycle progression beyond the G1/S phase transition requires the activation of a transcription complex containing histone nuclear factor P (HiNF-P) and nuclear protein mapped to ataxia telangiectasia (p220(NPAT)) in response to cyclin dependent kinase 2 (CDK2)/cyclin E signaling. We show here that the potent co-activating properties of HiNF-P/p220(NPAT) on the histone H4 gene promoter, which are evident in the majority of human cell types, are sporadically neutralized in distinct somatic cell lines. In cells where HiNF-P and p220(NPAT) do not activate the H4 gene promoter, HiNF-P instead represses transcription. Our data suggest that the cell type specific expression of the cyclin-dependent kinase inhibitory (CKI) protein p57(KIP2) inhibits the HiNF-P dependent activation of the histone H4 promoter. We propose that, analogous to E2F proteins and other cell cycle regulatory proteins, HiNF-P is a bifunctional transcriptional regulator that can activate or repress cell cycle controlled genes depending on the cellular context. |
| Links |
PubMed Online version:10.1002/jcb.21157 |
| Keywords |
Animals; COS Cells; Cell Cycle; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Line; Cell Line, Transformed; Cell Line, Tumor; Cell Transformation, Viral; Cercopithecus aethiops; Cyclin-Dependent Kinase Inhibitor p57/genetics; Cyclin-Dependent Kinase Inhibitor p57/metabolism; Genes, Reporter; HeLa Cells; Histones/genetics; Humans; Luciferases/metabolism; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Promoter Regions, Genetic; Repressor Proteins/genetics; Repressor Proteins/metabolism; Transcription, Genetic; Transfection |
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