PMID:17122336
| Citation |
Inoue, T, Shingaki, R, Hirose, S, Waki, K, Mori, H and Fukui, K (2007) Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J. Bacteriol. 189:950-7 |
|---|---|
| Abstract |
Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain. |
| Links |
PubMed PMC1797309 Online version:10.1128/JB.01294-06 |
| Keywords |
Antigens, Bacterial/genetics; Antigens, Bacterial/physiology; Citric Acid Cycle/genetics; Citric Acid Cycle/physiology; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli/ultrastructure; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/physiology; Fimbriae, Bacterial/chemistry; Fimbriae, Bacterial/genetics; Fimbriae, Bacterial/physiology; Gene Deletion; Genes, Bacterial; Genome, Bacterial; Glucose/metabolism; Microscopy, Electron, Transmission; Molecular Chaperones/genetics; Molecular Chaperones/physiology; Polysaccharides, Bacterial/metabolism; Protein Folding; Signal Transduction/genetics; Signal Transduction/physiology |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
