PMID:17119270
| Citation |
Kwon, D, Yoon, S, Carter, O, Bailey, GS and Dashwood, RH (2006) Antioxidant and antigenotoxic activities of Angelica keiskei, Oenanthe javanica and Brassica oleracea in the Salmonella mutagenicity assay and in HCT116 human colon cancer cells. Biofactors 26:231-44 |
|---|---|
| Abstract |
Epidemiological studies indicate that consumption of green-yellow vegetables rich in chlorophyll, vitamin C, vitamin E, and carotenoids reduce the risk of cancer. We sought to examine the antigenotoxic and antioxidant properties of chlorophyll-rich methanol extracts of Angelica keiskei, Oenanthe javanica, and Brassica oleracea (kale). In the Salmonella mutagenicity assay, A. keiskei caused dose-dependent inhibition against three heterocyclic amine mutagens in the presence of S9, O. javanica was antimutagenic only at the highest concentration in the assay (2 mg/plate), and B. oleracea showed no consistent inhibitory activity at non-toxic levels. None of the extracts were effective against three direct-acting mutagens in the absence of S9. Extracts of A. keiskei and, to a lesser extent O. javanica, inhibited two of the major enzymes that play a role in the metabolic activation of heterocyclic amines, based on ethoxyresorufin-O-deethylase and methoxyresorufin-O-demethylase assays in vitro. All three plant extracts were highly effective in assays which measured ferric reducing/antioxidant power, oxygen radical absorbance capacity, and Fe2+/H2O2-mediated DNA nicking. Finally, using the 'comet' assay, all three plant extracts protected against H2O2-induced genotoxic damage in human HCT116 colon cancer cells. These findings provide support for the antigenotoxic and antioxidant properties of chlorophyll-rich extracts of A. keiskei, O. javanica, and B. oleracea, through mechanisms that include inhibition of carcinogen activation and scavenging of reactive oxygen species. |
| Links | |
| Keywords |
Angelica/chemistry; Antimutagenic Agents/pharmacology; Antioxidants/pharmacology; Brassica/chemistry; Colon/drug effects; Colon/metabolism; Colonic Neoplasms; Cytochrome P-450 Enzyme System/antagonists & inhibitors; Cytochrome P-450 Enzyme System/metabolism; DNA Breaks, Single-Stranded; Free Radical Scavengers/therapeutic use; Heterocyclic Compounds/toxicity; Humans; Mutagenicity Tests; Oenanthe/chemistry; Phytotherapy; Plant Extracts/chemistry; Plant Extracts/pharmacology; Reactive Oxygen Species/metabolism; Salmonella typhimurium/drug effects; Tumor Cells, Cultured |
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