PMID:17105995
| Citation |
Barkan, A, Klipcan, L, Ostersetzer, O, Kawamura, T, Asakura, Y and Watkins, KP (2007) The CRM domain: an RNA binding module derived from an ancient ribosome-associated protein. RNA 13:55-64 |
|---|---|
| Abstract |
The CRS1-YhbY domain (also called the CRM domain) is represented as a stand-alone protein in Archaea and Bacteria, and in a family of single- and multidomain proteins in plants. The function of this domain is unknown, but structural data and the presence of the domain in several proteins known to interact with RNA have led to the proposal that it binds RNA. Here we describe a phylogenetic analysis of the domain, its incorporation into diverse proteins in plants, and biochemical properties of a prokaryotic and eukaryotic representative of the domain family. We show that a bacterial member of the family, Escherichia coli YhbY, is associated with pre-50S ribosomal subunits, suggesting that YhbY functions in ribosome assembly. GFP fused to a single-domain CRM protein from maize localizes to the nucleolus, suggesting that an analogous activity may have been retained in plants. We show further that an isolated maize CRM domain has RNA binding activity in vitro, and that a small motif shared with KH RNA binding domains, a conserved "GxxG" loop, contributes to its RNA binding activity. These and other results suggest that the CRM domain evolved in the context of ribosome function prior to the divergence of Archaea and Bacteria, that this function has been maintained in extant prokaryotes, and that the domain was recruited to serve as an RNA binding module during the evolution of plant genomes. |
| Links |
PubMed PMC1705760 Online version:10.1261/rna.139607 |
| Keywords |
Amino Acid Motifs; Cell Nucleus/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Evolution, Molecular; Molecular Sequence Data; Phylogeny; Plant Proteins/chemistry; Plant Proteins/classification; Plant Proteins/metabolism; Protein Structure, Tertiary/genetics; Protein Structure, Tertiary/physiology; RNA/chemistry; RNA/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/classification; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Ribosomes/chemistry; Ribosomes/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.