PMID:17060320
| Citation |
Frenkiel-Krispin, D, Wolf, SG, Albeck, S, Unger, T, Peleg, Y, Jacobovitch, J, Michael, Y, Daube, S, Sharon, M, Robinson, CV, Svergun, DI, Fass, D, Tzfira, T and Elbaum, M (2007) Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-VirE2 complex assembly by VirE1. J. Biol. Chem. 282:3458-64 |
|---|---|
| Abstract |
Agrobacterium tumefaciens infects plant cells by the transfer of DNA. A key factor in this process is the bacterial virulence protein VirE2, which associates stoichiometrically with the transported single-stranded (ss) DNA molecule (T-strand). As observed in vitro by transmission electron microscopy, VirE2-ssDNA readily forms an extended helical complex with a structure well suited to the tasks of DNA protection and nuclear import. Here we have elucidated the role of the specific molecular chaperone VirE1 in regulating VireE2-VirE2 and VirE2-ssDNA interactions. VirE2 alone formed functional filamentous aggregates capable of ssDNA binding. In contrast, co-expression with VirE1 yielded monodisperse VirE1-VirE2 complexes. Cooperative binding of VirE2 to ssDNA released VirE1, resulting in a controlled formation mechanism for the helical complex that is further promoted by macromolecular crowding. Based on this in vitro evidence, we suggest that the constrained volume of the VirB channel provides a natural site for the exchange of VirE2 binding from VirE1 to the T-strand. |
| Links |
PubMed Online version:10.1074/jbc.M605270200 |
| Keywords |
Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/physiology; Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/physiology; Bacterial Proteins/ultrastructure; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA, Single-Stranded/chemistry; DNA, Single-Stranded/metabolism; DNA-Binding Proteins/antagonists & inhibitors; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/ultrastructure; Ion Channels/antagonists & inhibitors; Ion Channels/biosynthesis; Ion Channels/metabolism; Ion Channels/ultrastructure; Molecular Chaperones/biosynthesis; Molecular Chaperones/genetics; Molecular Chaperones/physiology; Multiprotein Complexes/chemistry; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Plants/genetics; Plants/microbiology; Protein Binding/genetics; Protein Denaturation/genetics; Protein Structure, Secondary/genetics; Transformation, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.