PMID:17047874

Citation	Wing, C, Errey, JC, Mukhopadhyay, B, Blanchard, JS and Field, RA (2006) Expression and initial characterization of WbbI, a putative D-Galf:alpha-D-Glc beta-1,6-galactofuranosyltransferase from Escherichia coli K-12. Org. Biomol. Chem. 4:3945-50
Abstract	Cloning of E. coli K-12 orf8 (wbbI) and over-expression of the corresponding enzyme as a maltose-binding fusion protein provided recombinant WbbI beta-1,6-galactofuranosyltransferase activity. Challenged with synthetic acceptor analogues in the presence of UDP-galactofuranose as a donor, WbbI showed a modest preference for pyranoside acceptor substrates of the alpha-D-gluco-configuration but it also possessed the ability to turn-over acceptor analogues.
Links	PubMed Online version:10.1039/b609455d
Keywords	Chromatography, Thin Layer; Escherichia coli/enzymology; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/metabolism; Glycosyltransferases/isolation & purification; Glycosyltransferases/metabolism; Oligosaccharides/chemistry; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Substrate Specificity; Time Factors
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