PMID:1699845
| Citation |
Schoenlein, PV, Gallman, LS, Winkler, ME and Ely, B (1990) Nucleotide sequence of the Caulobacter crescentus flaF and flbT genes and an analysis of codon usage in organisms with G + C-rich genomes. Gene 93:17-25 |
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| Abstract |
The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulate flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5' ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3' region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop structure followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons preferred by C. crescentus. |
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| Keywords |
Amino Acid Sequence; Base Composition; Base Sequence; Cloning, Molecular; Codon; DNA, Bacterial/genetics; Flagellin/genetics; Genes, Bacterial; Gram-Negative Bacteria/genetics; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Messenger/genetics; Restriction Mapping; Transcription, Genetic |
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