PMID:1696718
| Citation |
Greenberg, JT, Monach, P, Chou, JH, Josephy, PD and Demple, B (1990) Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87:6181-5 |
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| Abstract |
Escherichia coli responds to superoxide-generating agents by inducing approximately 40 proteins. We have identified a genetic locus, soxR (superoxide response), that positively regulates 9 of these proteins during superoxide stress. Induction under soxR control is at the transcriptional level, as shown with lac fusions to five paraquat-inducible promoters. Members of the soxR regulon include at least three proteins with demonstrable antioxidant roles: Mn-containing superoxide dismutase (which destroys superoxide radicals), endonuclease IV (which repairs radical-induced damages in DNA), and glucose-6-phosphate dehydrogenase (which produces NADPH). Induction of the soxR regulon also leads to diminished levels of the major outer membrane protein OmpF and alteration of the small-subunit ribosomal protein S6. These latter changes confer resistance to a variety of antibiotics. The soxR regulon may thus operate as an inducible defense against xenobiotics in general. |
| Links | |
| Keywords |
Anti-Bacterial Agents/pharmacology; Antioxidants/pharmacology; Chromosome Deletion; Cloning, Molecular; DNA Transposable Elements; DNA-(Apurinic or Apyrimidinic Site) Lyase; Deoxyribonuclease IV (Phage T4-Induced); Drug Resistance, Microbial/genetics; Endodeoxyribonucleases/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Glucosephosphate Dehydrogenase/genetics; Methylnitronitrosoguanidine/pharmacology; Operon; R Factors; Superoxide Dismutase/genetics; Superoxides/metabolism; Superoxides/pharmacology; Transduction, Genetic |
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