PMID:1691706
| Citation |
Levitz, R, Chapman, D, Amitsur, M, Green, R, Snyder, L and Kaufmann, G (1990) The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease. EMBO J. 9:1383-9 |
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| Abstract |
The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon nuclease, an enzyme which triggers the cleavage-ligation of the host tRNALys. We report here the molecular cloning, nucleotide sequence and mutational analysis of prr-associated DNA. The results indicate that prr encodes a latent form of anticodon nuclease consisting of a core enzyme and cognate masking agents. They suggest that the T4-encoded factors of anticodon nuclease counteract the prr-encoded masking agents, thus activating the latent enzyme. The encoding of a tRNA cleavage-ligation pathway by two separate genetic systems which cohabitate E. coli may provide a clue to the evolution of RNA splicing mechanisms mediated by proteins. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Mutational Analysis; Enzyme Activation/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial/genetics; Molecular Sequence Data; RNA Splicing/genetics; RNA, Bacterial/metabolism; Ribonucleases/genetics; Ribonucleases/metabolism; T-Phages/genetics |
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