PMID:1691299
| Citation |
Faubladier, M, Cam, K and Bouché, JP (1990) Escherichia coli cell division inhibitor DicF-RNA of the dicB operon. Evidence for its generation in vivo by transcription termination and by RNase III and RNase E-dependent processing. J. Mol. Biol. 212:461-71 |
|---|---|
| Abstract |
We have established that the long non-coding intercistronic region of the dicB operon of Escherichia coli expresses a trans-acting division inhibitor specified by a region dicF, at most 65 nucleotides-long. The present study deals with the processing of dicBF operon mRNA in vivo, and identifies the dicF gene product as a 53 nucleotide RNA species. A sequence at the end of DicF resembles, and behaves as, a Rho-independent terminator, but further processing of readthrough transcripts, presumably by RNase III, followed by a limited 3' to 5' degradation, appears to generate additional DicF-RNA 3' ends. For the 5' end of DicF-RNA, our results show that a 190 nucleotide precursor DicF-RNA species is formed by cleavage at an RNase III site, while the 53 nucleotide minimal DicF-RNA is generated by further processing requiring the presence of an active form of RNase E in vivo. These data indicate that an untranslated product derived from an operon RNA can have a regulatory activity by affecting cell division. |
| Links |
PubMed Online version:10.1016/0022-2836(90)90325-G |
| Keywords |
Base Sequence; Cell Division; Endoribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli Proteins; Introns; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Operon; RNA Processing, Post-Transcriptional; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; Ribonuclease III; Transcription, Genetic |
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