PMID:16895982

Citation	Kutuzova, GD, Akhter, S, Christakos, S, Vanhooke, J, Kimmel-Jehan, C and Deluca, HF (2006) Calbindin D(9k) knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level. Proc. Natl. Acad. Sci. U.S.A. 103:12377-81
Abstract	Since the discovery of calbindin D(9k), its role in intestinal calcium absorption has remained unsettled. Further, a wide distribution of calbindin D(9k) among tissues has argued for its biological importance. We discovered a frameshift deletion in the calbindin D(9k) gene in an ES cell line, E14.1, that originated from 129/OlaHsd mice. We produced mice with the mutant calbindin D(9k) gene by injecting the E14.1 ES cell subline into the C57BL/6 host blastocysts and proved that these mice lack calbindin D(9k) protein. Calbindin D(9k) knockout mice were indistinguishable from wild-type mice in phenotype, were able to reproduce, and had normal serum calcium levels. Thus, calbindin D(9k) is not required for viability, reproduction, or calcium homeostasis.
Links	PubMed PMC1567887 Online version:10.1073/pnas.0605252103
Keywords	Animals; Base Sequence; Calcium/blood; Calcium-Binding Protein, Vitamin D-Dependent/genetics; Calcium-Binding Protein, Vitamin D-Dependent/metabolism; Cell Line; Frameshift Mutation; Mice; Mice, Inbred Strains; Mice, Knockout; Molecular Sequence Data; Phenotype
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