PMID:16771842
| Citation |
Gao, J, Lee, K, Zhao, M, Qiu, J, Zhan, X, Saxena, A, Moore, CJ, Cohen, SN and Georgiou, G (2006) Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. Mol. Microbiol. 61:394-406 |
|---|---|
| Abstract |
In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition--and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2006.05246.x |
| Keywords |
Endoribonucleases/genetics; Endoribonucleases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Polyribonucleotide Nucleotidyltransferase/genetics; Polyribonucleotide Nucleotidyltransferase/metabolism; Protein Disulfide-Isomerases/genetics; Protein Disulfide-Isomerases/metabolism; Protein Interaction Mapping; Protein Structure, Tertiary; RNA Helicases/genetics; RNA Helicases/metabolism; RNA Stability; RNA, Messenger/metabolism; Surface Plasmon Resonance |
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