PMID:16707683
| Citation |
Schönert, S, Seitz, S, Krafft, H, Feuerbaum, EA, Andernach, I, Witz, G and Dahl, MK (2006) Maltose and maltodextrin utilization by Bacillus subtilis. J. Bacteriol. 188:3911-22 |
|---|---|
| Abstract |
Bacillus subtilis can utilize maltose and maltodextrins that are derived from polysaccharides, like starch or glycogen. In this work, we show that maltose is taken up by a member of the phosphoenolpyruvate-dependent phosphotransferase system and maltodextrins are taken up by a maltodextrin-specific ABC transporter. Uptake of maltose by the phosphoenolpyruvate-dependent phosphotransferase system is mediated by maltose-specific enzyme IICB (MalP; synonym, GlvC), with an apparent K(m) of 5 microM and a V(max) of 91 nmol . min(-1) . (10(10) CFU)(-1). The maltodextrin-specific ABC transporter is composed of the maltodextrin binding protein MdxE (formerly YvdG), with affinities in the low micromolar range for maltodextrins, and the membrane-spanning components MdxF and MdxG (formerly YvdH and YvdI, respectively), as well as the energizing ATPase MsmX. Maltotriose transport occurs with an apparent K(m) of 1.4 microM and a V(max) of 4.7 nmol . min(-1) . (10(10) CFU)(-1). |
| Links |
PubMed PMC1482931 Online version:10.1128/JB.00213-06 |
| Keywords |
Bacillus subtilis/metabolism; Biological Transport; Genotype; Kinetics; Maltose/metabolism; Operon; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Plasmids; Polysaccharides/chemistry; Polysaccharides/metabolism; Restriction Mapping |
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