PMID:16677314
| Citation |
Giel, JL, Rodionov, D, Liu, M, Blattner, FR and Kiley, PJ (2006) IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O2-regulated genes in Escherichia coli. Mol. Microbiol. 60:1058-75 |
|---|---|
| Abstract |
IscR is an iron-sulphur (Fe-S) cluster-containing transcription factor that represses transcription of the operon containing its own gene and the iscSUA-hscBA-fdx genes, whose products are involved in Fe-S cluster biogenesis. In this study, global transcriptional profiling of Escherichia coli IscR(+) and IscR(-) strains grown under aerobic and anaerobic conditions indicated that 40 genes in 20 predicted operons were regulated by IscR. DNase I footprinting and/or in vitro transcription reactions identified seven new promoters under direct IscR control. Among these were genes encoding known or proposed functions in Fe-S cluster biogenesis (sufABCDSE, yadR and yhgI) and Fe-S cluster-containing anaerobic respiratory enzymes (hyaABCDEF, hybOABCDEFG and napFDAGHBC). The finding that IscR repressed expression of the hyaA, hybO and napF promoters specifically under aerobic growth conditions suggests a new mechanism to explain their upregulation under anaerobic growth conditions. Phylogenetic footprinting of the DNase I protected regions of seven promoters implies that there are at least two different classes of IscR binding sites conserved among many bacteria. The findings presented here indicate a more general role of IscR in the regulation of Fe-S cluster biogenesis and that IscR contributes to the O(2) regulation of several promoters controlling the expression of anaerobic Fe-S proteins. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2006.05160.x |
| Keywords |
Anaerobiosis/genetics; Base Sequence; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial/genetics; Iron-Sulfur Proteins/genetics; Molecular Sequence Data; Multigene Family/genetics; Oxidative Stress/genetics; Oxygen/physiology; Promoter Regions, Genetic/genetics; Superoxide Dismutase/genetics; Transcription Factors/genetics; Transcription Factors/metabolism |
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