PMID:1658833
| Citation |
Stokes, HW and Hall, RM (1991) Sequence analysis of the inducible chloramphenicol resistance determinant in the Tn1696 integron suggests regulation by translational attenuation. Plasmid 26:10-9 |
|---|---|
| Abstract |
The sequence of the Tn1696 determinant for inducible nonenzymatic chloramphenicol resistance has been determined. The cml region, the fourth insert of the Tn1696 integron, is 1547 bases and includes a 59-base element at the 3' end, as is typical of integron inserts. One gene, designated cmlA and predicting a polypeptide of 44.2 kDa, is encoded in the insert. However, the cmlA region shows one feature not previously found in an integron insert. A promoter is located within the cmlA insert, and translational attenuation signals related to those of the inducible cat and ermC genes found in gram-positive organisms are also present. The regulatory region includes a leader peptide of nine amino acids, a ribosome stall sequence related to those preceding cat genes, and two alternative pairs of stem-loop structures which either sequester or disclose the ribosome binding site and start codon preceding the cmlA gene. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Chloramphenicol/pharmacology; Cloning, Molecular; DNA Transposable Elements; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Drug Resistance, Microbial/genetics; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Molecular Sequence Data; Nucleic Acid Conformation; Plasmids; Protein Biosynthesis; Protein Sorting Signals/genetics; RNA, Messenger/genetics; Restriction Mapping; Sequence Homology, Nucleic Acid |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
This paper identifies a 9aa leader peptide for regulation of cmr expression.
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.