PMID:1656379
| Citation |
Zuerner, RL (1991) Physical map of chromosomal and plasmid DNA comprising the genome of Leptospira interrogans. Nucleic Acids Res. 19:4857-60 |
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| Abstract |
The size and physical structure of the Leptospira interrogans genome was characterized using contour-clamped homogenous electric field (CHEF) gel electrophoresis. The L. interrogans genome is approximately 4750 kb in size and is composed of two molecular species of DNA: a 4400 kb chromosome; and a 350 kb plasmid, pLIN1. A physical map of the chromosome was constructed with the restriction enzymes NotI and SfiI. A physical map of pLIN1 was constructed with ApaI, NotI, Sse83871, SgrAI, and SmaI. Both the L. interrogans chromosome and pLIN1 are circular. |
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| Keywords |
Chromosome Mapping; Cloning, Molecular; DNA Restriction Enzymes/metabolism; DNA, Bacterial/genetics; Genes, Bacterial; Leptospira interrogans/genetics; Nucleic Acid Hybridization; Plasmids; Restriction Mapping |
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