PMID:1655701
| Citation |
Oden, KL and Gennis, RB (1991) Isolation and characterization of a new class of cytochrome d terminal oxidase mutants of Escherichia coli. J. Bacteriol. 173:6174-83 |
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| Abstract |
Cytochrome d terminal oxidase mutants were isolated by using hydroxylamine mutagenesis of pNG2, a pBR322-derived plasmid containing the wild-type cyd operon. The mutagenized plasmid was transformed into a cyo cyd recA strain, and the transformants were screened for the inability to confer aerobic growth on nonfermentable carbon sources. Western blot analysis and visible-light spectroscopy were performed to characterize three independent mutants grown both aerobically and anaerobically. The mutational variants of the cytochrome d complex were stabilized under anaerobic growth conditions. All three mutations perturb the b595 and d heme components of the complex. These mutations were mapped and sequenced and are shown to be located in the N-terminal third of subunit II of the cytochrome d complex. It is proposed that the N terminus of subunit II may interact with subunit I to form an interface that binds the b595 and d heme centers. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cytochrome d Group; Cytochromes/genetics; Electron Transport Chain Complex Proteins; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Gene Expression; Genetic Markers; Molecular Sequence Data; Mutagenesis; Oxidoreductases/classification; Oxidoreductases/genetics; Oxidoreductases/isolation & purification; Plasmids |
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