PMID:16551743

Citation	Park, JS and Roberts, JW (2006) Role of DNA bubble rewinding in enzymatic transcription termination. Proc. Natl. Acad. Sci. U.S.A. 103:4870-5
Abstract	By using DNA heteroduplexes that inhibit rewinding of the upstream part of the transcription bubble, we show that transcript release in termination by the enzymes Mfd and Rho is facilitated by reannealing of DNA in the upstream region of the transcription bubble, as is also true for termination by intrinsic terminators. We also show that, like Mfd, the Rho termination factor promotes forward translocation of RNA polymerase. These results support termination models in which external forces imposed on nucleic acids induce concerted rewinding of DNA and unwinding of the DNA/RNA hybrid, possibly accompanied by forward translocation of RNA polymerase, leading to transcription complex dissociation.
Links	PubMed PMC1405909 Online version:10.1073/pnas.0600145103
Keywords	Bacterial Proteins/metabolism; Biological Transport; DNA/chemistry; DNA/genetics; DNA/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Molecular Sequence Data; Nucleic Acid Conformation; RNA/genetics; RNA/metabolism; Terminator Regions, Genetic/genetics; Transcription Factors/metabolism; Transcription, Genetic/genetics
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