PMID:1653212
| Citation |
Hammond, GG, Cassidy, PJ and Overbye, KM (1991) Novobiocin-dependent topA deletion mutants of Escherichia coli. J. Bacteriol. 173:5564-7 |
|---|---|
| Abstract |
Previous reports of the transduction of topA deletions in Escherichia coli suggested that delta top A transductants grow normally only if they acquire spontaneous mutations that compensate for the topoisomerase I defect. We show that P1-mediated transduction of delta topA in the presence of sublethal concentrations of novobiocin, an inhibitor of the DNA gyrase B subunit, yields uncompensated Top- isolates which are dependent on novobiocin for optimum growth. In the absence of novobiocin these delta topA strains grow slowly, indicating that topA deletions are deleterious but not lethal to the cell. We propose that inhibitors of DNA gyrase B, presumably by lowering intracellular levels of DNA supercoiling, can phenotypically suppress a topoisomerase I defect in E. coli. |
| Links | |
| Keywords |
Chromosome Deletion; DNA Topoisomerases, Type I/genetics; DNA Topoisomerases, Type I/metabolism; Drug Resistance, Microbial/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes, Bacterial; Mutation; Novobiocin/metabolism; Transduction, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.