PMID:16522795
| Citation |
Marani, P, Wagner, S, Baars, L, Genevaux, P, de Gier, JW, Nilsson, I, Casadio, R and von Heijne, G (2006) New Escherichia coli outer membrane proteins identified through prediction and experimental verification. Protein Sci. 15:884-9 |
|---|---|
| Abstract |
Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five-YftM, YaiO, YfaZ, CsgF, and YliI--and also provide preliminary data indicating that a sixth--YfaL--may be an outer membrane autotransporter. |
| Links |
PubMed PMC2242486 Online version:10.1110/ps.051889506 |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Outer Membrane Proteins/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Fractionation; Cloning, Molecular; Computational Biology/methods; Escherichia coli/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/isolation & purification; Escherichia coli Proteins/metabolism; Membrane Transport Proteins/genetics; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Proteome/chemistry; Proteome/genetics; Proteome/metabolism |
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