PMID:1650909
| Citation |
Itaya, M and Crouch, RJ (1991) Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H. Mol. Gen. Genet. 227:433-7 |
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| Abstract |
The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)]. |
| Links | |
| Keywords |
Alleles; Endoribonucleases/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Mutation; Nucleic Acid Hybridization; Phenotype; Plasmids; Ribonuclease H; Temperature |
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