PMID:16490983
| Citation |
Müller, G, Hai, DN and Kramer, A (2006) Lack of in vitro genotoxicity of povidone-iodine in solution, in ointment or in a liposomal formulation (Repithel). Dermatology (Basel) 212 Suppl 1:94-7 |
|---|---|
| Abstract |
The in vitro genotoxicity of povidone-iodine in Repithel, Betaisodona ointment and Betaisodona solution was investigated using CHO-K1 cells. The comet assay and chromosome aberration test were used to characterise the genotoxic potency of povidone-iodine within 4 h of contact with test cells. Methyl methanesulphonate without S9 activation and benzo[a]pyrene with S9 activation were used as positive controls. No higher concentrations than 6% Repithel, 5% Betaisodona ointment and 2% Betaisodona solution in culture medium with 10% fetal bovine serum were tested within 4 h of contact with CHO-K1 cells for the comet assay, because higher concentrations will provide >60% cytotoxicity. All tested concentrations of povidone-iodine formulations (4-6% Repithel, 3-5% Betaisodona ointment and 1-2% Betaisodona solution) are not genotoxic in the comet assay. 10% Repithel, 5% Betaisodona ointment and 2.5% Betaisodona solution in culture medium with 10% fetal bovine serum were final concentrations in the chromosome aberration test, which provide <60% cytotoxicity within 4 h of contact with CHO-K1 cells. All tested concentrations of povidone- iodine formulations (5-10% Repithel, 2.5-5% Betaisodona ointment and 1.25-2.5% Betaisodona solution) are not genotoxic in the chromosome aberration test. Using the comet assay and chromosome aberration test to characterise the genotoxic potency of povidone-iodine within 4 h of contact with CHO-K1 cells, there was no chromosome damage detected. |
| Links |
PubMed Online version:10.1159/000090103 |
| Keywords |
Animals; Anti-Infective Agents, Local/administration & dosage; Anti-Infective Agents, Local/toxicity; CHO Cells/drug effects; Chromosome Aberrations/chemically induced; Comet Assay; Cricetinae; Cricetulus; Liposomes; Mutagenicity Tests; Ointments; Pharmaceutical Solutions; Povidone-Iodine/administration & dosage; Povidone-Iodine/toxicity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.